Human capital development and economic sustainability linkage in Sub-Saharan African countries: Novel evidence from augmented mean group approach.

Sustainable Development Goal number four (SDG-4) strongly emphasizes quality education, which is crucial to human capital development. The importance of human capital development for sustainable economic development has thus risen to the top of the global policy agenda. However, the empirical literature on the topic has several limitations, including utilizing inappropriate measurement of human capital development and being unable to investigate the quality dimensions of education. Therefore, this study used years of schooling and return on education to fill the gap, and also considered the role of institutional and political factors in its empirical estimation. The main objective of this study is to investigate the effect of human capital development on economic sustainability in 30 Sub-Saharan African countries, employing panel data from 2000 to 2020. The augmented mean group model was used in the empirical investigation. The findings of the study showed that human capital development negatively and significantly affects economic sustainability in Sub-Saharan African countries. Thus, among the policy alternatives that Sub-Saharan African countries and policymakers should pursue to attain the goals of economic sustainability is revisiting the entire human capital development architecture and putting more of an emphasis on quality education than on access to education.

Household Splitting Process and Food Security in Malawi.

Despite the frequent changes in household composition in Sub-Saharan Africa, the literature on the household division process is sparse, with no evidence of its effect on food security. This paper addresses the topic in Malawi, where the fission process is evident and malnutrition is a severe problem. Using the Integrated Household Panel Dataset, this study applies the difference-in-difference model with the propensity score matching technique to compare matched groups of households that did and did not split between 2010 and 2013. The results suggest that coping strategies adopted by poor households and life course events determine household fission in Malawi, a process that benefits household food security in the short term. On average, the food consumption score is 3.74 units higher among households that split between 2010 and 2013 compared to the matched households that did not. However, the household division might have long-run adverse effects on food insecurity, especially for poor households due to the adoption of coping strategies that might compromise their human capital and income-generating activities. Therefore, this process warrants attention for the more accurate understanding, design, and evaluation of food security interventions.

Impact of Climate Variables Change on the Yield of Wheat and Rice Crops in Iran (Application of Stochastic Model Based on Monte Carlo Simulation).

This study aims to predict the yield of two strategic crops in Iran; wheat and rice, under climate scenarios that indicate probable changes in climate variables. It implemented by a stochastic model based on the Monte Carlo method. This model were estimated based on historical data from 1988 to 2019 for precipitation and temperature provided possible changes in the pattern of and their probability of occurrence. The results show that rain-fed wheat, irrigated wheat and rice yields decrease by 42%, 29% and 21% respectively in the average scenario. Therefore, policy makers should try to make the right decisions to increase the production of the country's strategic crops. R&D management to introduce drought-tolerant varieties, use of modern irrigation systems and use of low-volume irrigation methods are some of the proposed solutions to mitigate the effects of climate change.

Economic evaluation of the climate changes on food security in Iran: application of CGE model.

The present study aims to examine the economic impact of changing climate variables on two components of food security in Iran: availability and access to food. Wheat and rice, the two most important foods in the country, were considered representatives of food security. A CGE model was developed to achieve the research goals. In this context, a stochastic model based on Monte Carlo simulation was used to provide three scenarios (best, average, and worst) indicating probable changes in climate variables. It is important to model the problem of changing climatic variables for irrigated crops, as groundwater resource depletion and restrictions on extraction from Iranian aquifers reduce planted areas and yields. Therefore, this study applies this model to both rain-fed and irrigated crops, whereas studies in the literature only evaluate rain-fed crops. Food security will face serious challenges as food supplies, and consumption of goods and services are declining in average and worst scenarios, according to findings. Consequently, the negative impact of climate change on food security and people's livelihoods requires a review of the policies implemented within the country. Effective solutions include research and development to introduce drought-tolerant varieties and adopt appropriate strategies to adapt to climate change. Improving the incomes of farmers is one solution to mitigating the impacts of climate change.

Conflict and Child Malnutrition: a Systematic Review of the Emerging Quantitative Literature.

PURPOSE OF REVIEW: Reduction of child malnutrition in conflict settings is on top of the international agenda on sustainable development. This association between child malnutrition and conflict has been hypothesised in the academic literature but not rigorously examined empirically till recently. This paper reviews the emerging quantitative literature, including conflict as an explanatory variable to understand the aspects of child malnutrition studied and how violent events are associated with child nutritional status. Limitations are also highlighted. RECENT FINDINGS: Child malnutrition is investigated into its three main dimensions of stunting, wasting and undernourishment computed as z-score. Conflict is mostly studied in terms of duration, number of events, typology and intensity. The emerging literature generally establishes a significant and negative association between conflict and child malnutrition. However, limitations persist and are mainly due to the type of available data.

A latent growth curve modelling approach to seasonal and spatial dynamics of food security heterogeneities in rural Lake Naivasha Basin, Kenya.

The increasing complexity of food insecurity, malnutrition, and chronic poverty faced by Sub-Saharan Africa warrants urgent categorisation and tracking of household food security along both temporal and spatial dimensions. This will help to effectively target, monitor and evaluate population-level programs and specific interventions aimed at addressing food insecurity. Traditional longitudinal analysis does not address the dynamics of inter- and intrahousehold heterogeneities within the seasonal and spatial context of household-level food security. This study is the first to overcome such limitations by adopting a multi-group piecewise latent growth curve model in the analysis of the food security situation in a statistically representative sample of 601 households involved in subsistence and cut-flower commercial agriculture, around Lake Naivasha. We considered food security as a latent concept, which manifests as food security outcomes in our primary longitudinal dataset from March 2018 to January 2019. Our analysis highlights the temporal and spatial dynamics of food security and advances new evidence on inter- and intrahousehold heterogeneities in food security across different seasons for the subsistence and commercial farming clusters. These heterogeneities were demonstrated primarily during the hunger season from March to June, and persisted in both the clusters and across months, albeit with different intensities. Moreover, our results indicate the importance of commercial agriculture in achieving food security in the hunger season. Our study suggests the need of a multidisciplinary approach to food security and the introduction of well-coordinated interventions for the development of subsistence and commercial agriculture considering the seasonal and cluster-level specificities.

In vitro dexamethasone treatment does not induce alternative ATM transcripts in cells from Ataxia-Telangiectasia patients.

Short term treatment with low doses of glucocorticoid analogues has been shown to ameliorate neurological symptoms in Ataxia-Telangiectasia (A-T), a rare autosomal recessive multisystem disease that mainly affects the cerebellum, immune system, and lungs. Molecular mechanisms underlying this clinical observation are unclear. We aimed at evaluating the effect of dexamethasone on the induction of alternative ATM transcripts (ATMdexa1). We showed that dexamethasone cannot induce an alternative ATM transcript in control and A-T lymphoblasts and primary fibroblasts, or in an ATM-knockout HeLa cell line. We also demonstrated that some of the reported readouts associated with ATMdexa1 are due to cellular artifacts and the direct induction of γH2AX by dexamethasone via DNA-PK. Finally, we suggest caution in interpreting dexamethasone effects in vitro for the results to be translated into a rational use of the drug in A-T patients.

Haploidentical hematopoietic stem cell transplantation in adults using the αßTCR/CD19-based depletion of G-CSF-mobilized peripheral blood progenitor cells.

In patients with hematological malignancies at high risk for relapse, a mismatched hematopoietic stem cells transplants can be offered with no undue delay between decision-making and transplantation as virtually all patients have a full-haplotype mismatched member who could serve immediately as a donor. Using a T-cell depletion approach, these patients can benefit from a graft-vs-leukemia effect in the absence of both acute and chronic graft-vs-host disease. Over the past decade, efforts have concentrated on developing new conditioning regimens, optimizing the graft processing and improving the posttransplant immunological recovery. The innovative strategy based on the selective depletion of alpha/beta-positive T lymphocytes from G-CSF-mobilized peripheral blood precursor cells has shown very promising results in the setting of the pediatric transplantation. This paper reports the outcome in adult patients with hematological malignancies.

Comparison of Volumetric and Bead-Based Counting of CD34 Cells by Single-Platform Flow Cytometry.

BACKGROUND: Over 2,000 people a year in the United Kingdom need a bone marrow or blood stem cell transplant. It is important to accurately quantify the hematopoietic stem cells to predict whether the transplant will be successful in replenishing the immune system. However, they are present at low frequency, which complicates accurate quantification. The current gold standard method is single-platform flow cytometry using internal reference counting beads to determine the concentration of CD34 cells. However, volumetric flow cytometers have the ability to measure the acquisition volume, which removes the need for reference beads for calculation of cell concentrations. METHOD: In this study, we compared both methods for calculating CD34 cell concentrations in volumetric cytometers, using either the volume reading or the number of reference beads for calculation. In addition, the uncertainty of measurement for each method was estimated. RESULTS: The results show that both methods have similar uncertainties of measurement. Regression analysis showed low to no statistical difference in CD34 cell concentrations obtained with each method. CONCLUSIONS: Overall, this study suggests that the volumetric method is a valid approach but that the adoption of this technology may be hindered without some form of external calibration of volume readings to increase confidence in the measurement. © 2019 The Authors. Cytometry Part B: Clinical Cytometry published by Wiley Periodicals, Inc. on behalf of International Clinical Cytometry Society.

Cellular Metrology: Scoping for a Value Proposition in Extra- and Intracellular Measurements.

The symptomatic irreproducibility of data in biomedicine and biotechnology prompts the need for higher order measurements of cells in their native and near-native environments. Such measurements may support the adoption of new technologies as well as the development of research programs across different sectors including healthcare and clinic, environmental control and national security. With an increasing demand for reliable cell-based products and services, cellular metrology is poised to help address current and emerging measurement challenges faced by end-users. However, metrological foundations in cell analysis remain sparse and significant advances are necessary to keep pace with the needs of modern medicine and industry. Herein we discuss a role of metrology in cell and cell-related R&D activities to underpin growing international measurement capabilities. Relevant measurands are outlined and the lack of reference methods and materials, particularly those based on functional cell responses in native environments, is highlighted. The status quo and current challenges in cellular measurements are discussed in the light of metrological traceability in cell analysis and applications (e.g., a functional cell count). An emphasis is made on the consistency of measurement results independent of the analytical platform used, high confidence in data quality vs. quantity, scale of measurements and issues of building infrastructure for end-users.

Assessment of Digital PCR as a Primary Reference Measurement Procedure to Support Advances in Precision Medicine.

BACKGROUND: Genetic testing of tumor tissue and circulating cell-free DNA for somatic variants guides patient treatment of many cancers. Such measurements will be fundamental in the future support of precision medicine. However, there are currently no primary reference measurement procedures available for nucleic acid quantification that would support translation of tests for circulating tumor DNA into routine use. METHODS: We assessed the accuracy of digital PCR (dPCR) for copy number quantification of a frequently occurring single-nucleotide variant in colorectal cancer (KRAS c.35G>A, p.Gly12Asp, from hereon termed G12D) by evaluating potential sources of uncertainty that influence dPCR measurement. RESULTS: Concentration values for samples of KRAS G12D and wild-type plasmid templates varied by <1.2-fold when measured using 5 different assays with varying detection chemistry (hydrolysis, scorpion probes, and intercalating dyes) and <1.3-fold with 4 commercial dPCR platforms. Measurement trueness of a selected dPCR assay and platform was validated by comparison with an orthogonal method (inductively coupled plasma mass spectrometry). The candidate dPCR reference measurement procedure showed linear quantification over a wide range of copies per reaction and high repeatability and interlaboratory reproducibility (CV, 2%-8% and 5%-10%, respectively). CONCLUSIONS: This work validates dPCR as an SI-traceable reference measurement procedure based on enumeration and demonstrates how it can be applied for assignment of copy number concentration and fractional abundance values to DNA reference materials in an aqueous solution. High-accuracy measurements using dPCR will support the implementation and traceable standardization of molecular diagnostic procedures needed for advancements in precision medicine.

Compact devices for generation of reference trace VOC mixtures: a new concept in assuring quality at chemical and biochemical laboratories.

Volatile organic compounds (VOCs) in gas mixtures at trace level (nmol/mol) are routinely measured by chemical and biochemical laboratories as climate indicators, indoor air quality pollutants from building materials emissions, contaminants in food and beverages, and biomarkers in body fluids (blood, urine, breath) of occupational exposure or human diseases. Current analytical instruments used for measurements are gas chromatographs equipped with various injector and detector configurations. The assurance of measurement quality is done by using a huge amount of certified liquid VOC standard solutions (or gaseous VOC standard cylinders) with multiple dilutions to reach the required trace level. This causes high standard uncertainty in instrument calibrations, high cost, and high consumption of analysis and laboratory personal time. In this paper, we present the implementation of portable generators producing VOC gas standards at trace level for automatic and direct calibration of VOC detectors employed in various contexts, removing the need for preparation of matrix calibration standards in cylinders. Two compact devices in-house developed by two national metrology institutes-the Istituto Nazionale di Ricerca Metrologica (INRIM) and the Federal Institute of Metrology (METAS)-are here used to dynamically generate reference gas mixtures in an SI traceable way. The two devices are based on different technologies: diffusion and permeation, for INRIM and METAS, respectively. A metrological characterization is given and the practical implementation at chemical and biochemical laboratories is discussed. Graphical abstract Onsite calibration with transportable generation system with similar performances to primary laboratory devices.

Uso de elastômero para delimitação do término do preparo em modelos odontológicos / Use of elastomer for delimitation of the preparation terminal edge in dental models

A comunicação entre o dentista e o técnico de prótese dentária é fundamental para o tratamento. A técnica convencional de troquelização de modelos consiste em sua individualização na região dos preparos através do recorte e eliminação do gesso fora do espaço correspondente ao sulco gengival na região do término. O objetivo deste artigo é descrever dois casos clínicos nos quais foi utilizada uma técnica inovadora de troquelização de modelos.

Communication between the dentist and the dental prosthesis technician is fundamental for treatments. The conventional technique of die casting models consists of their individualization in the preparations region, through plaster cutting and elimination outside the space corresponding to the gingival sulcus in the terminal region. The aim of this article is to describe two clinical cases in which an innovative technique of die casting models was used.

Droplet volume variability as a critical factor for accuracy of absolute quantification using droplet digital PCR.

Accurate and precise nucleic-acid quantification is crucial for clinical and diagnostic decisions, as overestimation or underestimation can lead to misguided treatment of a disease or incorrect labelling of the products. Digital PCR is one of the best tools for absolute nucleic-acid copy-number determination. However, digital PCR needs to be well characterised in terms of accuracy and sources of uncertainty. With droplet digital PCR, discrepancies between the droplet volume assigned by the manufacturer and measured by independent laboratories have already been shown in previous studies. In the present study, we report on the results of an inter-laboratory comparison of different methods for droplet volume determination that is based on optical microscopy imaging and is traceable to the International System of Units. This comparison was conducted on the same DNA material, with the examination of the influence of parameters such as droplet generators, supermixes, operators, inter-cartridge and intra-cartridge variability, and droplet measuring protocol. The mean droplet volume was measured using a QX200™ AutoDG™ Droplet Digital™ PCR system and two QX100™ Droplet Digital™ PCR systems. The data show significant volume differences between these two systems, as well as significant differences in volume when different supermixes are used. We also show that both of these droplet generator systems produce droplets with significantly lower droplet volumes (13.1%, 15.9%, respectively) than stated by the manufacturer and previously measured by other laboratories. This indicates that to ensure precise quantification, the droplet volumes should be assessed for each system.

Synthesis, structure and non-linear optical properties of new isostructural ß-D-fructopyranose alkaline halide metal-organic frameworks: a theoretical and an experimental study.

In this work four metal-organic framework isomorphs, based on fructose and alkali-earth halogenides, were investigated to better understand the effect of the size of the cation and the different polarizability of the anion on the calculated hyperpolarizability and optical susceptibility, which are correlated to non-linear optical properties. The compounds were characterized by X-ray diffraction and the first hyperpolarizability and the second-order susceptibility were obtained from theoretical calculations. Furthermore, a new method to measure the second-harmonic (SH) efficiency on a small quantity of powder at different wavelengths of excitation was optimized and an attempt was made to assess the reduction of the SH intensity for small quantities of nano-crystals, in order to ascertain the possibility of applications in biological systems. The results of this work show that both the intrinsic nature of the anion and the induced dissociation of cations and anions by fructose play a role in the second-harmonic generating properties of such compounds.

Immune tolerance induction by nonmyeloablative haploidentical HSCT combining T-cell depletion and posttransplant cyclophosphamide.

The establishment of safe approaches to attain durable donor-type chimerism and immune tolerance toward donor antigens represents a major challenge in transplantation biology. Haploidentical hematopoietic stem cell transplantation (HSCT) is currently used for cancer therapy either as a T-cell-depleted megadose HSCT following myeloablative conditioning or with T-cell-replete HSCT following nonmyeloablative conditioning (NMAC) and high-dose posttransplant cyclophosphamide (PTCY). The latter approach suffers from a significant rate of chronic graft-versus-host disease (GVHD), despite prolonged immunosuppression. The use of T-depleted grafts, although free of GVHD risk, is not effective after NMAC because of graft rejection. We now demonstrate in mice conditioned with NMAC that combining the power of high-dose PTCY with T-cell-depleted megadose HSCT can overcome this barrier. This approach was evaluated in 2 patients with multiple myeloma and 1 patient with Hodgkin lymphoma. The first myeloma patient now followed for 25 months, exhibited full donor-type chimerism in the myeloid and B-cell lineages and mixed chimerism in the T-cell compartment. The second myeloma patient failed to attain chimerism. Notably, the low toxicity of this protocol enabled a subsequent successful fully myeloablative haploidentical HSCT in this patient. The third patients was conditioned with slightly higher total body irradiation and engrafted promptly. All patients remain in remission without GVHD. Both engrafted patients were able to control cytomegalovirus reactivation. Enzyme-linked immunospot analysis revealed immune tolerance toward donor cells. Our results demonstrate a novel and safer nonmyeloablative haplo-HSCT offering a platform for immune tolerance induction as a prelude to cell therapy and organ transplantation.

Micro- and Macrostructured PLGA/Gelatin Scaffolds Promote Early Cardiogenic Commitment of Human Mesenchymal Stem Cells In Vitro.

The biomaterial scaffold plays a key role in most tissue engineering strategies. Its surface properties, micropatterning, degradation, and mechanical features affect not only the generation of the tissue construct in vitro, but also its in vivo functionality. The area of myocardial tissue engineering still faces significant difficulties and challenges in the design of bioactive scaffolds, which allow composition variation to accommodate divergence in the evolving myocardial structure. Here we aimed at verifying if a microstructured bioartificial scaffold alone can provoke an effect on stem cell behavior. To this purpose, we fabricated microstructured bioartificial polymeric constructs made of PLGA/gelatin mimicking anisotropic structure and mechanical properties of the myocardium. We found that PLGA/gelatin scaffolds promoted adhesion, elongation, ordered disposition, and early myocardial commitment of human mesenchymal stem cells suggesting that these constructs are able to crosstalk with stem cells in a precise and controlled manner. At the same time, the biomaterial degradation kinetics renders the PLGA/gelatin constructs very attractive for myocardial regeneration approaches.

Quantification of cells with specific phenotypes I: determination of CD4+ cell count per microliter in reconstituted lyophilized human PBMC prelabeled with anti-CD4 FITC antibody.

A surface-labeled lyophilized lymphocyte (sLL) preparation has been developed using human peripheral blood mononuclear cells prelabeled with a fluorescein isothiocyanate conjugated anti-CD4 monoclonal antibody. The sLL preparation is intended to be used as a reference material for CD4+ cell counting including the development of higher order reference measurement procedures and has been evaluated in the pilot study CCQM-P102. This study was conducted across 16 laboratories from eight countries to assess the ability of participants to quantify the CD4+ cell count of this reference material and to document cross-laboratory variability plus associated measurement uncertainties. Twelve different flow cytometer platforms were evaluated using a standard protocol that included calibration beads used to obtain quantitative measurements of CD4+ T cell counts. There was good overall cross-platform and counting method agreement with a grand mean of the laboratory calculated means of (301.7 ± 4.9) µL(-1) CD4+ cells. Excluding outliers, greater than 90% of participant data agreed within ±15%. A major contribution to variation of sLL CD4+ cell counts was tube to tube variation of the calibration beads, amounting to an uncertainty of 3.6%. Variation due to preparative steps equated to an uncertainty of 2.6%. There was no reduction in variability when data files were centrally reanalyzed. Remaining variation was attributed to instrument specific differences. CD4+ cell counts obtained in CCQM-P102 are in excellent agreement and show the robustness of both the measurements and the data analysis and hence the suitability of sLL as a reference material for interlaboratory comparisons and external quality assessment.

A mechanical characterization of polymer scaffolds and films at the macroscale and nanoscale.

Biomaterials should be mechanically tested at both the nanoscale and macroscale under conditions simulating their working state, either in vitro or in vivo, to confirm their applicability in tissue engineering applications. In this article, polyester-urethane-based films and porous scaffolds produced by hot pressing and thermally induced phase separation respectively, were mechanically characterized at both the macroscale and nanoscale by tensile tests and indentation-type atomic force microscopy. All tests were conducted in wet state with the final aim of simulating scaffold real operating conditions. The films showed two distinct Young Moduli populations, which can be ascribed to polyurethane hard and soft segments. In the scaffold, the application of a thermal cooling gradient during phase separation was responsible for a nanoscale polymer chain organization in a preferred direction. At the macroscale, the porous matrices showed a Young Modulus of about 1.5 MPa in dry condition and 0.3 MPa in wet state. The combination of nanoscale and macroscale values as well as the aligned structure are in accordance with stiffness and structure required for scaffolds used for the regeneration of soft tissues such as muscles.

A first approach to evaluate the cell dose in highly porous scaffolds by using a nondestructive metabolic method.

BACKGROUND: In cell-based therapies, in vitro studies on biomimetic cell-scaffold constructs can facilitate the determination of the cell dose, a key factor in guaranteeing the effectiveness of the treatment. However, highly porous scaffolds do not allow a nondestructive evaluation of the cell number. Our objective was to develop a nondestructive method for human mesenchymal stem cells dose evaluation in a highly porous scaffold for bone regeneration. MATERIALS & MEASUREMENT METHOD: Proliferation trend of human mesenchymal stem cells on Biocoral® scaffolds was measured by a resazurin-based assay here optimized for 3D cultures. The method allows to noninvasively follow the cell proliferation on biocorals over 3 weeks with very high reproducibility. CONCLUSION: This reliable method could be a powerful tool in cell-based therapies for cell dose determination.